RESEARCH PROBLEM OF TUBERCULOCIDAL ACTIVITY OF DISINFECTANTS

Disinfection of surfaces, medical instruments and medical waste, contaminated with mycobacteria of tuberculosis is currently the most important issue in the health facility of TB profile.

Today tuberculocidal activity of disinfectants is tested on the test culture Mycobacterium terrae.

BACKGROUND

Mycobacteria - thin rod-shaped cells with a characteristic property of the acid and alcohol resistance (one of the stages of growth), aerobic. On Gram stain they are weakly Gram-positive.

Mycolic acids are present as free sulfolipids and cord factor, the presence of which on surfaces is associated with the cell characteristic colony formation of M. tuberculosis in a harness form and pathogenicity.

Mycobacterium terrae – saprophyte non-patogenic non-tuberculous mycobacteria; the main habitat - soil and water.

The cell wall contains no cord-factor, but it contains a large amount of mineral elements, which makes it more resistant to adverse environmental factors!

PROBLEM

Many reputable experts of TB services suggest that the study of tuberculocidal activity on strain of Mycobacterium terrae is not conclusively proven tuberculocidal activity. Research is needed on the efficacy against clinical isolates of tuberculosis mycobacteria.

Research should be undertaken in respect of the virulent clinical isolates with varying degrees of resistance to anti-TB drugs, as follows:

  • N37RV - (h - the laboratory, sensitive to all anti-TB drugs)

  • Sensitivity-1 and Sensitivity-2 - sensitive to anti-TB drugs clinical (wild) strains isolated from diagnostic material of patients with pulmonary tuberculosis.

  • MDR - clinical, steady

  • XDR - clinical, steady

MDR TB - multidrug -resistant tuberculosis, which is caused by strains of tuberculosis mycobacteria resistant to the two main anti-TB drugs - isoniazid and rifampicin. To combat them, special and in most cases highly toxic drugs are required that cause serious side effects.

XDR TB - multidrug-resistant tuberculosis, which is caused by strains of tuberculosis mycobacteria, resistant not only to isoniazid and rifampicin, but also to kanamycin and fluoroquinolone group (ofloxacin, levofloxacin, maksakvin, etc.)

Works with bacteria 1-2 Pathogenicity can be performed only in institutions having access to work with such pathogen. Investigations are conducted jointly with the Central Research Institute of Tuberculosis of RAMS.

Currently there was proved tuberculocidal activity of preparations OPTIMAKS and DIABAK on virulent clinical strains with varying degrees of resistance to anti-TB drugs.

There were also identified working (effective) concentrations recommended for use in health care facilities of TB services .